in fusion hd cloning kit (TaKaRa)
TaKaRa is a verified supplier 
TaKaRa manufactures this product 
99
Structured Review
TaKaRa
in fusion hd cloning kit
In Fusion Hd Cloning Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 10117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/in+fusion+hd+cloning+system/In-Fusion+HD+Cloning+Kit/pmc13123328-130-12-16
Average 99 stars, based on 10117 article reviews
In Fusion Hd Cloning Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 10117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/in+fusion+hd+cloning+system/In-Fusion+HD+Cloning+Kit/pmc13123328-130-12-16
Average 99 stars, based on 10117 article reviews
in fusion hd cloning kit - by Bioz Stars,
2026-09
99/100 stars
Images
Related Articles
Sequencing:Article Title: Neuroprotective gene therapy targeting the AKT pathway Article Snippet: AAV Vectors A plasmid encoding the human AKT3 cDNA sequence containing N-terminal myristoylation (MYR) and HA tags was kindly provided by William Sellers (addgene plasmid #9017). .. The MYR-HA-hAKT3 sequence was amplified and cloned into an AAV proviral expression plasmid using the Article Title: Efficient and accurate prime editing system in plants. Article Snippet: .. Rice codon-optimized M-MLV RT coding sequence (SV40 NLS_engineered M-MLV RT (D200N/L603W/T330P/T306K/ W313F) (Anzalone et al., 2019)_SV40 NLS) was synthesized by GeneArt Gene Synthesis and introduced into the KpnI site located at the C-terminal region of nSpCas9-N863A using the Article Title: Mitochondrial calcium shapes B cell signaling and mitochondrial function Article Snippet: .. Duplicated mitochondrial targeting sequence (MTS) and GCaMP6s were amplified and inserted to linearized pMIG empty vector via Article Title: Efficient and accurate prime editing system in plants. Article Snippet: .. The resulting fragments (the 20-nt pegRNA protospacer:: original or modified scaffold sequence::RT template::PBS::8-nt linker) were introduced into the BbsI site of pOsU6_BbsIx2_tQ1_ polyT using the Article Title: Efficient and accurate prime editing system in plants. Article Snippet: .. The ABE8e_nSpCas9-NG coding sequence was digested with SmaI/KpnI from the pPZP_ZmUbi:ABE8e_nSpCas9-NG vector and was replaced with a rice codon-optimized SpCas9 coding sequence (Mikami et al., 2015) amplified by PCR using the primer sets listed in Table S1 using the Amplification:Article Title: Neuroprotective gene therapy targeting the AKT pathway Article Snippet: AAV Vectors A plasmid encoding the human AKT3 cDNA sequence containing N-terminal myristoylation (MYR) and HA tags was kindly provided by William Sellers (addgene plasmid #9017). .. The MYR-HA-hAKT3 sequence was amplified and cloned into an AAV proviral expression plasmid using the Article Title: Mitochondrial calcium shapes B cell signaling and mitochondrial function Article Snippet: .. Duplicated mitochondrial targeting sequence (MTS) and GCaMP6s were amplified and inserted to linearized pMIG empty vector via Article Title: Efficient and accurate prime editing system in plants. Article Snippet: .. The ABE8e_nSpCas9-NG coding sequence was digested with SmaI/KpnI from the pPZP_ZmUbi:ABE8e_nSpCas9-NG vector and was replaced with a rice codon-optimized SpCas9 coding sequence (Mikami et al., 2015) amplified by PCR using the primer sets listed in Table S1 using the Clone Assay:Article Title: Neuroprotective gene therapy targeting the AKT pathway Article Snippet: AAV Vectors A plasmid encoding the human AKT3 cDNA sequence containing N-terminal myristoylation (MYR) and HA tags was kindly provided by William Sellers (addgene plasmid #9017). .. The MYR-HA-hAKT3 sequence was amplified and cloned into an AAV proviral expression plasmid using the Article Title: Characterization of the Regulatory AAA-ATPase Subunit Rpt3 in Plasmodium berghei as an Activator of Protein Phosphatase 1. Article Snippet: .. The coding regions of PbRpt3-45 kDa (a.a. 1–395) and PbRpt3-30 kDa (a.a. 131–395) were obtained via PCR with primers P1–P2 and P2–P4, respectively (Supplementary Table S2) and cloned into pETDuet-1 (Novagen) using the Bioprocessing:Article Title: Neuroprotective gene therapy targeting the AKT pathway Article Snippet: AAV Vectors A plasmid encoding the human AKT3 cDNA sequence containing N-terminal myristoylation (MYR) and HA tags was kindly provided by William Sellers (addgene plasmid #9017). .. The MYR-HA-hAKT3 sequence was amplified and cloned into an AAV proviral expression plasmid using the Expressing:Article Title: Neuroprotective gene therapy targeting the AKT pathway Article Snippet: AAV Vectors A plasmid encoding the human AKT3 cDNA sequence containing N-terminal myristoylation (MYR) and HA tags was kindly provided by William Sellers (addgene plasmid #9017). .. The MYR-HA-hAKT3 sequence was amplified and cloned into an AAV proviral expression plasmid using the Plasmid Preparation:Article Title: Neuroprotective gene therapy targeting the AKT pathway Article Snippet: AAV Vectors A plasmid encoding the human AKT3 cDNA sequence containing N-terminal myristoylation (MYR) and HA tags was kindly provided by William Sellers (addgene plasmid #9017). .. The MYR-HA-hAKT3 sequence was amplified and cloned into an AAV proviral expression plasmid using the Article Title: Mitochondrial calcium shapes B cell signaling and mitochondrial function Article Snippet: .. Duplicated mitochondrial targeting sequence (MTS) and GCaMP6s were amplified and inserted to linearized pMIG empty vector via Cloning:Article Title: Neuroprotective gene therapy targeting the AKT pathway Article Snippet: AAV Vectors A plasmid encoding the human AKT3 cDNA sequence containing N-terminal myristoylation (MYR) and HA tags was kindly provided by William Sellers (addgene plasmid #9017). .. The MYR-HA-hAKT3 sequence was amplified and cloned into an AAV proviral expression plasmid using the Article Title: Efficient and accurate prime editing system in plants. Article Snippet: .. Rice codon-optimized M-MLV RT coding sequence (SV40 NLS_engineered M-MLV RT (D200N/L603W/T330P/T306K/ W313F) (Anzalone et al., 2019)_SV40 NLS) was synthesized by GeneArt Gene Synthesis and introduced into the KpnI site located at the C-terminal region of nSpCas9-N863A using the Article Title: Mitochondrial calcium shapes B cell signaling and mitochondrial function Article Snippet: .. Duplicated mitochondrial targeting sequence (MTS) and GCaMP6s were amplified and inserted to linearized pMIG empty vector via Article Title: Efficient and accurate prime editing system in plants. Article Snippet: .. The resulting fragments (the 20-nt pegRNA protospacer:: original or modified scaffold sequence::RT template::PBS::8-nt linker) were introduced into the BbsI site of pOsU6_BbsIx2_tQ1_ polyT using the Article Title: Functional Characterization of OTU Domain-Containing Deubiquitinases from Plant Pathogenic Fungi Reveals Distinct Immune Modulatory Mechanisms Article Snippet: Concurrently, the pET-26b(+) vector (Novagen, Madison, WI, USA) was linearized with the same two restriction enzymes. .. Each insert was then ligated into the linearized pET-26b(+) backbone via the Article Title: Characterization of the Regulatory AAA-ATPase Subunit Rpt3 in Plasmodium berghei as an Activator of Protein Phosphatase 1. Article Snippet: .. The coding regions of PbRpt3-45 kDa (a.a. 1–395) and PbRpt3-30 kDa (a.a. 131–395) were obtained via PCR with primers P1–P2 and P2–P4, respectively (Supplementary Table S2) and cloned into pETDuet-1 (Novagen) using the Article Title: Inducible, split base editors for in vivo cancer functional genomics. Article Snippet: Vector construction and sgRNA cloning PCR fragments were amplified with Phusion High-Fidelity DNA Polymerase (Thermo Scientific). .. Vectors were generated using the Article Title: Efficient and accurate prime editing system in plants. Article Snippet: .. The ABE8e_nSpCas9-NG coding sequence was digested with SmaI/KpnI from the pPZP_ZmUbi:ABE8e_nSpCas9-NG vector and was replaced with a rice codon-optimized SpCas9 coding sequence (Mikami et al., 2015) amplified by PCR using the primer sets listed in Table S1 using the Synthesized:Article Title: Efficient and accurate prime editing system in plants. Article Snippet: .. Rice codon-optimized M-MLV RT coding sequence (SV40 NLS_engineered M-MLV RT (D200N/L603W/T330P/T306K/ W313F) (Anzalone et al., 2019)_SV40 NLS) was synthesized by GeneArt Gene Synthesis and introduced into the KpnI site located at the C-terminal region of nSpCas9-N863A using the Construct:Article Title: Mitochondrial calcium shapes B cell signaling and mitochondrial function Article Snippet: .. Duplicated mitochondrial targeting sequence (MTS) and GCaMP6s were amplified and inserted to linearized pMIG empty vector via Modification:Article Title: Efficient and accurate prime editing system in plants. Article Snippet: .. The resulting fragments (the 20-nt pegRNA protospacer:: original or modified scaffold sequence::RT template::PBS::8-nt linker) were introduced into the BbsI site of pOsU6_BbsIx2_tQ1_ polyT using the Polymerase Chain Reaction:Article Title: Characterization of the Regulatory AAA-ATPase Subunit Rpt3 in Plasmodium berghei as an Activator of Protein Phosphatase 1. Article Snippet: .. The coding regions of PbRpt3-45 kDa (a.a. 1–395) and PbRpt3-30 kDa (a.a. 131–395) were obtained via PCR with primers P1–P2 and P2–P4, respectively (Supplementary Table S2) and cloned into pETDuet-1 (Novagen) using the Article Title: Efficient and accurate prime editing system in plants. Article Snippet: .. The ABE8e_nSpCas9-NG coding sequence was digested with SmaI/KpnI from the pPZP_ZmUbi:ABE8e_nSpCas9-NG vector and was replaced with a rice codon-optimized SpCas9 coding sequence (Mikami et al., 2015) amplified by PCR using the primer sets listed in Table S1 using the Generated:Article Title: Inducible, split base editors for in vivo cancer functional genomics. Article Snippet: Vector construction and sgRNA cloning PCR fragments were amplified with Phusion High-Fidelity DNA Polymerase (Thermo Scientific). .. Vectors were generated using the |